Possible reasons for poor root growth in tissue culture plugs and the corresponding solutions:
| Problem | Possible Reasons | Solutions |
|---|---|---|
| Insufficient Hormones | - Low levels of auxins and cytokinins | - Adjust hormone concentrations in the media |
| - Degraded or expired hormone solutions | - Use fresh, high-quality growth regulators | |
| Inadequate Lighting | - Insufficient light intensity | - Increase light levels or extend photoperiod |
| - Inadequate light spectrum for rooting | - Use appropriate light spectrum (e.g., blue light) | |
| High Humidity | - Excessive moisture on the plugs | - Improve ventilation and reduce misting |
| - Contamination by fungi or bacteria | - Sterilise tools and media, maintain strict sterile practices | |
| Incorrect pH | - Media pH is too acidic or alkaline | - Adjust media pH to the optimal range (usually 5.5 - 6.5) |
| Overcrowding | - Plugs placed too closely, limiting aeration | - Space plugs appropriately and use larger containers |
| Suboptimal Temperature | - Unsuitable temperatures for root initiation | - Maintain the appropriate temperature for the specific plant species |
| - Temperature fluctuations | - Create a controlled environment or provide insulation | |
| Poor Media | - Low nutrient content or improper composition | - Ensure media contains essential nutrients and minerals |
| - Compacted media | - Loosen the media to enhance aeration | |
| Contaminated Tools | - Tools not adequately sterilised | - Thoroughly sterilise tools and containers |
| - Presence of pathogenic microorganisms on tools | - Regularly clean and sanitise equipment | |
| Genetic Factors | - Genetic factors affecting root formation | - Use tissue cultures from healthy, well-rooted plants |
Remember to adapt these solutions to your specific ornamental indoor plant species and growing conditions in the UK.
